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| Thanks to the following for materials & support |
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Microelectrodes
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| For details on our work with microelectrodes, see the following projects and also refer to the protocols. |
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| • Microsensors based on enzymes >> |
| • The fabrication and use of ultramicroelectrode sensors >> |
| • Hybrid sensors >> |
| • Ultra-micro oxygen sensor development >> |
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| Garber, S.S., Messerli, M.A., Hubert, M., Lewis, R., Hammar, K., Indyk, E. and Smith, P.J.S. 2005. Monitoring Cl- movement in single cells exposed to hypotonic solution. Journal of Membrane Biology, 203:101-110. |
| Osbourn, D.M., Sanger, R. H., and Smith, P.J.S. 2005. Determination of single cell oxygen consumption with impedance feedback for control of sample-probe separation. Analytical Chemistry, 77(21):6999-7004. |
| Messerli, M.A., Smith, P.J.S., Lewis, R.C., Robinson, K.R. 2004. Chloride fluxes in lily pollen tubes: a critical reevaluation. Plant Journal, 40:799-812. |
| Bogorff, D.J., Messerli, M.A., Malchow, R.P. and Smith, P.J.S. 2003. Development and characterization of a self-referencing glutamate-selective micro biosensor. Biological Bulletin, 205: 207-208. |
| Cooper, R.A. and Jung, S. 2002. Single cell electrochemistry. In: Encyclopedia of Electrochemistry: 9 (Biochemistry), GS Wilson (ed). Wiley & Sons Publishers. |
| Jung, S.-K., Trimarchi, J.R., Sanger, R.H. and Smith, P.J.S. 2001. Development and application of a self-referencing glucose microsensor for the measurement of glucose consumption by pancreatic beta cells. Analytical Chemistry, 73(15): 3759-3767. |
| Porterfield, D.M., Laskin, J.D., Jung, S.-K., Malchow, R.P., Billack, B., P.J.S. Smith and Heck, D.E. 2001. Proteins and lipids define the diffusional field of nitric oxide. American Journal of Physiology, 281(4): L904-912. |
| Smith, P.J.S. and Trimarchi, J.R. 2001. Noninvasive measurement of hydrogen and potassium ion flux from single cells and epithelial structures. American Journal of Physiology, 280: C1-C11. |
| Smith, P.J.S., Haydon, P.G., Hengstenberg, A. and Jung, S.-K. 2001. Analysis of cellular boundrary layers and their modulation by plasma membrane transporters: Application of electrochemical microsensors. Electrochimica Acta, 47(1-2): 283-292. |
| Jung, S.-K., Hammar, K. and Smith, P.J.S. 2000. Development of self-referencing oxygen microsensor and its application to HIT cells. Biological Bulletin, 199(2): 197-198. |
| Porterfield, D.M., Corkey, R.F., Sanger, R.H., Tornheim, K., Smith, P.J.S. and Corkey, B.E. 2000. Oxygen consumption oscillates in single clonal pancreatic beta -cells (HIT). Diabetes, 49: 1511-1516. |
| Land, S.C., Porterfield, D.M., Sanger, R.H. and Smith, P.J.S. 1999. The self-referencing oxygen-selective microelectrode: Detection of transmembrane oxygen flux from single cells. Journal of Experimental Biology, 202: 211-218. |
| Baikie, I.D., Smith, P.J.S., Porterfield, D.M. and Estrup, P.J. 1999. Multi-Tip Scanning Bio-Kelvin Probe. Review of Scientific Instruments, 70(3): 1842-1850. |
| Malchow, R.P., Land, S.C., Patel, L.S. and Smith, P.J.S. 1997. Consumption of oxygen by isolated skate retinal photoreceptors measured using a self-referencing oxygen-selective microelectrode. Biological Bulletin, 193: 231-232. |
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Equipment
