BioCurrents Research Center : Overview

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OVERVIEW

The BioCurrents Research Center (BRC) is an integrated technology resource of the NIH:NCRR. The activities of the Center focus on molecular physiology as it relates to the cell function and disease. Our particular interest is how the dynamics of cell responses are reflected in the chemical profiles of microdomains surrounding single living cells. In order to measure complex cellular boundary layers, the BRC has specialized in the development of extremely sensitive signal acquisition and processing methods along with miniaturized electrochemical sensor designs. The technique is non-invasive and termed self-referencing.

Cell structure is exceptionally complex and specialized & a lot is packed into a small package. Recent studies using high-resolution tomography (see Marsh et al, 2001: PNAS 98(5); 2399) graphically illustrate this at the cytosolic level, but structural and functional complexity extend to within the lipid bilayer and beyond into the microdomains of charge distribution and chemical profiles surrounding the cell membrane and glycocalyx & see figure opposite. A goal of the BRC is to design instrumentation and experimental approaches that allow investigators to study these domains and relate the data to normal function and pathophysiology.

History & Research Development
Since its establishment in 1996 the BRC has directed its technological research and development to the design and application of ultra-microelectrodes (tip diameters of less than 10µm) tailored for the detection of specific chemicals. These have been successfully applied to the boundary layer profiles of many different cell types, with thematic strength in diabetes research, reproductive health and development (see collaborative profiles). More recently, we are changing our focus to technical developments, enhancing the integrative approach to cell function.

To understand a cell as a dynamic and integrated whole, we must be able to examine responses from different domains as near to real time and as synchronously as possible. To this end, we are developing imaging capabilities to work in parallel with electrochemistry and conventional electrophysiological techniques. Imaging includes a spinning disc confocal, a commercial product from Perkin Elmer, as well as a low light/luminescent imager designed and built within the BRC. Click here for a complete inventory and description of equipment.

Outreach
The technologies developed or under development are in high demand within the biomedical community. Over 40 investigators work with the Center each year in a collaborative or service capacity. Over 80% of our visitor pool is NIH funded, representing approximately 25 NIH divisions and institutes. As part of our training and dissemination program we host occasional workshops at major national and international meetings, train a significant number of new investigators each year and host graduate students undertaking portions of their thesis dissertation using our technologies. In dissemination we advise on, and install, electrochemical systems in off campus research endeavors, both academic and industrial.