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| Thanks to the following for materials & support |
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Reproductive Health
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| Collaborators with the BRC have contributed significantly to our understanding of male and female reproductive physiology. Studies involved the utilization of the original ion-selective electrode approach, as well as our more recent amperometric, solid state, devices. Imaging has played an increasingly strong role and, through interactions with collaborative groups, molecular biology has contributed additional facets of knowledge. In this sense we have produced some exciting and truly integrated projects not possible anywhere else. |
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| • Glutamate excitotoxicity >> |
| • Mechanisms of luminal acidification in epidydimas & vas deferens >> |
• Flux studies from mammalian embryos as indicators of viability
& cell death >> |
| • Linking mitochondria & reproductive aging associated egg infertility >> |
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Beaulieu, V., Da Silva, N., Pastor-Soler, N., Brown, C.R., Smith, P.J.S., Brown, D., Breton, S. 2005. Modulation of the actin cytoskeleton via gelsolin regulates vacuolar H+-ATPase recycling. Journal of Biological Chemistry, 280:8452-8463.
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Li, R., Chase, M., Jung, S.-K., Smith, P.J.S., and Loeken, M.R. 2005. Hypoxic stress in diabetic pregnancy contributes to impaired embryo gene expression and defective development by inducing oxidative stress. American Journal of Physiology, Endocrinology and Metabolism, 289(4): E591-9.
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Pepperell JR, Porterfield D.M., Keefe DL, Behrman HR and Smith P.J.S. 2003. Control of ascorbic acid efflux in rat luteal cells: role of intracellular calcium and oxygen radicals. American Journal of Physiology. Cell Physiology. 285(3): C642-51.
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Liu, L., Trimarchi, J.R., Smith, P.J.S., Keefe, D.L. 2002. Mitochondrial dysfunction leads to telomere attrition and genomic instability. Aging Cell, 1:40-46.
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| Liu, L., Trimarchi, J.R. and Keefe, D.L. 2002. Haploidy but not parthenogenetic activation leads to increased incidence of apoptosis in mouse embryos. Biology of Reproduction, 66: 204-210. |
| Liu, L., Trimarchi, J.R., Smith, P.J.S. and Keefe, D.L. 2002. Checkpoint for DNA integrity at the first mitosis after oocyte activation. Molecular Reproduction and Development, 62: 277-288. |
| Trimarchi, J.R., Liu, L., Smith, P.J.S. and Keefe, D.L. 2002. Apoptosis recruits two-pore domain potassium channels used for homeostatic volume regulation. American Journal of Physiology. Cell Physiology. 282: C588-C594. |
| Liu, L., Hammar, K., Smith, P.J.S., Inoue, S., Keefe, D. 2001. Mitochondrial modulation of calcium signaling at the initiation of development. Cell Calcium, 30(6): 423-433. |
| Breton, S., Nsumu, N.N., Galli, T., Smith, P.J.S. and Brown, D. 2000. Tetanus toxin-mediated cleavage of cellubrevin impairs proton secretion in the male reproductive tract. American Journal of Physiology. Renal Physiology. 278(5): F717-725. |
| Herak-Kramberger, C., Sabolic, I., Blanusa, M., Smith, P.J.S., Brown, D. and Breton, S. 2000. Cadmium inhibits vacuolar H+ATPase-mediated acidification in rat epididymis. Biology of Reproduction, 63: 599-606. |
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Trimarchi, J.R., Liu, L., Smith, P.J.S. and Keefe, D.L. 2000. Non-invasive measurement of potassium efflux as an early indicator of cell death in mouse embryos. Biology of Reprodution, 63: 851-857. |
| Trimarchi, J.R., Liu, L., Porterfield, D.M., Smith, P.J.S. and Keefe, D.L. 2000. A non-invasive method for measuring pre-implantation embryo physiology. Zygote, 8: 15-24. |
| Trimarchi, J.R., Liu, L., Porterfield, D.M., Smith, P.J.S. and Keefe, D.L. 2000. Oxidative phosphorylation-dependent and -independent oxygen consumption by individual preimplantation mouse embryos. Biology of Reproduction, 62: 1866-1874. |
| Pepperell, J.R., Kommineni, K., Buradagunta, S., Smith, P.J.S. and Keefe, D.L. 1999. Transmembrane regulation of intracellular calcium by a plasma membrane Sodium/Calcium exchanger in mouse oocytes. Biology of Reproduction, 60: 1137-1143. |
| Hill, J. L., Hammar, K., Smith, P. J. S. and Gross, D. 1999. Stage-dependent effects of epidermal growth factor on Ca2+ efflux in mouse oocytes. Molecular Reproduction and Development, 53: 244-253. |
| Breton, S., Hammar, K., Smith, P.J.S. and Brown, D. 1998. Proton secretion in the male reproductive tract: involvement of chloride-independent bicarbonate transport. American Journal of Physiology, 275: C1134-C1142. |
| Porterfield, D.M., Trimarchi, J.R., Keefe, D.L. and Smith, P.J.S. 1998. Characterization of oxygen and calcium fluxes from early mouse embryos and oocytes. Biological Bulletin, 195: 208-209. |
| Breton, S., Smith, P.J.S. and Brown, D. 1996/7. Presence de cellules acidificantes dans l'epididyme et le canal deferent: implication de la pompe a protons, H+ATPase. Medecine Sciences (Revue internationale de biologie et de medicine). #5 (Mars-avril): 12-16. Also printed in same journal 1997, 13: 57-61. |
| Brown, D., Smith, P.J.S. and Breton, S. 1997. Role of V-ATPase rich cells in acidification of the male reproductive tract. Journal of Experimental Biology, 200 (2): 257-262. |
| Breton, S., Smith, P.J.S., Lui, B. and Brown, D. 1996. Acidification of the male reproductive tract by a proton-pumping ATPase. Nature Medicine, 2: 470-472. |
| Brown, D. and Breton, S. 1996. Mitochondria-rich, proton-secreting epithelial cells. Journal of Experimental Biology, 199(Pt 11): 2345-2358. |
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Equipment
