ELECTROCHEMISTRY & PHYSIOLOGY
SPINNING DISK CONFOCAL -
A Perkin Elmer spinning disk scanning unit (CSU-21) plus Orca CCD (Hamamatsu) is installed on a Zeiss Axiovert 200 inverted microscope with integrated electrochemical and electrochemistry micromanipulators and acquisition hardware. The multi-line argon laser (Melles Griot) accommodates 488, 568 and 647nm laser lines. Epifluorescence
is available on this system via
an illuminator (EXFO). It is housed on an air suspension table, in a darkened Faraday box, which can be heated to 37o
9 RESEARCH & EXPERIMENTAL PLATFORMS
which can be configured for self-referencing electrochemistry and standard patch / sharp microelectrode recording for current and voltage clamp. These include headstages and amplifiers from Molecular Devices as well as BRC headstages and controller units.
Four platforms also support FLUORESCENCE GLOBAL IMAGING
on 3 inverted and 1 upright Zeiss microscope. Micropositioning is performed with many different commercially available fine and coarse, 3-axis positioners including 4 fine controlled Narishige MHW-3 positioners.
MICROPIPETTE & MICROSENSOR FABRICATION:
The BRC has two Flaming/Brown micropipette pullers (P97; Sutter Instruments) and one laser-based puller (P2000; Sutter Instruments). These are housed in a dedicated clean room.
DIELECTROPHORESIS SYSTEM: The wave form generator for developing the dielectrophoretic system is already available at the BRC indeed it was designed and built there. This system is portable and can operated from any of the experimental platforms in BRC or the MBL Central Microscopy Facility.