BioCurrents Research Center : Protocols, Tutorials and Databases

Thanks to the following for materials & support

Protocols: Building the technology

~ Electrode construction: Potentiometric

~ Electrode construction: Amperometric

~ Electrode construction: Enzyme assisted

~ Data collection

~ Buffer solutions

~ Equipment and software

~ Data analysis

~ Spreadsheets (in progress)

See References

Tutorials: Utilizing the technology

~ Self referencing: the basics

~ Problems and pitfalls

~ Applications

See References

Databases

~ Pharmabase

~ Chemical inventory

See References

References

Sutter Instrument P-97 Pipette Cookbook
Download Cookbook (8,999 Kb)

Messerli, M.A., Kurtz, I. and Smith, P.J.S. 2008. Characterization of Optimized Na⁺ and Cl^- liquid membranes for use with extracellular, self-referencing microelectrodes. Anal. Bioanal. Chem. In Press.

Messerli, M.A., Corson, E.D., and Smith, P.J.S. 2007. Measuring extracellular ion gradients from single channels with ion-selective microelectrodes. Biophysical Journal. 92(7):L52-4.

Messerli, M.A., Robinson, K.R. 2007. MS Channels in Tip Growing Systems. Current Topics in Membranes. Vol. 58. Elsevier, Amsterdam. pp. 391-412.

Smith, P.J.S., Sanger, R.S. and Messerli, M.A. 2007. Principles, development and applications of self-referencing electrochemical microelectrodes to the determination of fluxes at cell membranes. In: Methods and New Frontiers in Neuroscience. Ed. Adrian C. Michael. CRC Press. Ch. 18: 373-405.

Smith, P.J.S. and Remsen, D. 2006. Using Pharmabase to Perform Pharmacological Analyses of Cell Function. In Current Protocols in Bioinformatics: Cheminformatics. Eds. Baxevanis, A., Davison,.D., Page, R., Petsko, G., Stormo, G. and Stein. L. John Wiley and Sons. Supplement 13, Section 14.2, 14.2.1 - 14.2.17.

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"If you don't know the answer, find the method." - Dr. John F. Moore